Biotech News - Kreatech Biotechnology BV Launches the First Complete kit for Whole Genome Ampli

Kreatech Biotechnology BV Launches the First Complete kit for Whole
Genome Amplification and Labeling for arrayCGH Analysis
Monday July 24, 5:33 am ET

Kreatech Biotechnology BV (www.kreatech.com) announces the launch of
the "Genome-pULSe, arrayCGH Whole Genome Amplification and Labeling
Kit" the latest addition to their product portfolio for arrayCGH
analysis.


This kit combines QIAGEN's whole genome amplification (WGA)
technology, which allows amplification of very small amounts of
genomic DNA isolated from tissue or cells, with KREATECH's ULS(TM)
reagents necessary to label these samples for arrayCGH analysis,
thereby offering a more complete solution to scientists carrying out
arrayCGH analysis. The Genome-pULSe Kit is available with the ULS
(TM) labeling molecule bound to Cy(TM)3 and Cy(TM)5 dyes licensed
from GE Healthcare. The WGA technology included in the kit is the
REPLI-g® kit from QIAGEN.

ArrayCGH analysis of particular samples, such as small needle tumor
biopsies, can be limited by the small amount of sample available.
Traditional methods of genomic DNA amplification include the time-
consuming process of creating EBV-transformed cell lines followed by
whole genome amplification using random or degenerate
oligonucleotide-primed PCR. However, all PCR-based methods can
generate non-specific amplification artefacts, resulting in the
incomplete coverage of loci. In addition to this, conventional
methods that are based on enzymatic incorporation of modified
nucleotides used to label the amplified DNA for subsequent
microarray analysis could further increase the bias in locus
representation.

The Genome-pULSe Kit delivers a novel procedure for the uniform
amplification and subsequent direct (non-enzymatic) labeling of
whole genome DNA from small samples. This method has been designed
to provide a quick and highly reproducible amplification and
labeling procedure for arrayCGH analysis.

"The combination of the ULS technology with REPLI-g in the Genome-
pULSe Kit enables us to deliver a unique product to researchers."
said Brent Keller, General Manager, Kreatech USA and VP Commercial
Operations, Kreatech Biotechnology BV. "It combines the advantage of
QIAGEN's Phi29 driven Whole Genome DNA Amplification with the
direct, non-enzymatic ULS labeling technology. With the unique
combination of ULS and REPLI-g, customers looking for amplification
of small input volumes of genomic DNA for microarray applications
will now be able to amplify and label directly for arrayCGH
analysis. This is the first amplification and labeling kit available
for arrayCGH analysis that enables customers to detect genetic
aberrations without introducing bias during the amplification and
labeling unlike conventional amplification and enzymatic labeling
procedures".

The Genome-pULSe kit further completes KREATECH's arrayCGH product
line. In June 2005 the company released the "ULS(TM) arrayCGH
Labeling Kits". The ULS arrayCGH labeling kit has the unique feature
of allowing the user to label genomic DNA directly in a one step
labeling reaction (30 minutes), avoiding the need for a potentially
biasing enzymatic step. This results in more accurate data as well
as saving time. The ULS labeling efficiency is independent of
fragment length making it ideal for use with archival DNA.

KREATECH's ULS technology relies on the special binding properties
of platinum to form coordinative bonds with biomolecules by binding
to specific sites on DNA, RNA and proteins. In this way, ULS acts as
relatively simple "molecular glue" for DNA, RNA and proteins. As a
result of its versatility, customers can attach any kind of
molecular label to ULS and then bind the labeled ULS complex to the
biomolecules of interest. Since the ULS technology does not require
the enzymatic incorporation of modified nucleotides into RNA or DNA,
it does not introduce bias.

QIAGEN's REPLI-g® technology is based on Multiple Displacement
Amplification, which carries out isothermal genome amplification
utilizing a uniquely processive DNA polymerase capable of
replicating 100 kb without dissociating from the genomic DNA
template. The DNA polymerase has a proofreading activity to maintain
high fidelity during replication and provide highly uniform "bias
free" DNA amplification across the entire genome.

About KREATECH

KREATECH Biotechnology B.V., a private company founded in 1990,
focuses on the creation, development, production and marketing of
innovative diagnostic and life science products. KREATECH's
proprietary Universal Linkage System (ULS(TM)) offers a reliable,
robust and easy to use non-enzymatic technology that can be applied
to a broad range of biomolecules and labeling agents. KREATECH is
committed to achieve and maintain the highest standards in
production and Quality Control as well as in the relationships with
our customers and partners. The company's quality policies are
demonstrated by the implementation of ISO 9001 (2000) and ISO 13485
(GMP 2003) guidelines. More information on KREATECH can be accessed
at www.kreatech.com.

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Source: Kreatech Biotechnology B.V.

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